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C57BL/6J mice were fed a standard chow diet for 12 weeks, then orally administered vehicle (n=10), or 10 mg/kg/day of MDMG-935P (n=8) for an additional 12 weeks on the standard chow diet prior to end point analyses

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Hanzlikova et al, 2018)

To quantify the mean florescence intensity (MFI) in each colon section in ImageJ software (NIH, Bethesda, MD), three cross sections for each mouse were analyzed with an optical microscope (magnification, 20)