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The addition of chalcone isomerase and chalcone reductase along with the flavanone pathway then led to the production of both 5-deoxyflavanones and 5-hydroxyflavanones (Chemler and Koffas, 2008)

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Upon interaction with GSH, IR806-PDA is converted into IR806-S-NH, resulting in a spectral shift (from 680 to 820 nm) and generating ratiometric photoacoustic signals that correlate with GSH levels

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