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For neutrophil sorting, APC-conjugated anti-CD45 (clone 30-F11, Catalog #559864, BD Biosciences), PE-conjugated anti-Ly6G (clone 1A8, Catalog #551481, BD Biosciences), and APC-Cy7-conjugated anti-CD11b (clone M1/70, Catalog #47-0112-82, eBioscience) were added and incubated for 30 min at 4 C in the dark

Please note that due to daylight savings time & different time zones the savings calculations for stopping smoking for periods less than a day might be slightly off, but the monetary savings grow more accurate over time & will accurately record each day on a forward basis

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In fact, the authors, troubled by the contradictions, revisited the issue in 2010, investigating several possible systematic biases that might explain them

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