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Implementing best practices is the next

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REM sleep deprivation causes an increase in noradrenaline (which incidentally causes the person sleep deprived to be stressed) due to the neurons in the locus coeruleus producing it not ceasing to do so, which causes an increase in the activity of the Na/K-ATPase pump, which itself activates the intrinsic pathway of apoptosis [48] and prevents autophagy, which also induces the mitochondrial pathway of apoptosis

K.AlthagafyH

The assay was performed as per the manufacturer's protocol (Affymetrix, San Diego, CA, USA)