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Wipe the rubber stoppers with alcohol and let them dry before each pierce, use a fresh sterile needle and syringe every time, and work in a clean preparation area

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PERK deficiency reduced the expression of RUNX2 and Osterix, master regulators of osteogenesis as well as important cell cycle factors, such as CCND, CCNE, CCNA, CDK1, and CDK2 leading to decreased cell proliferation [88] (Fig

The qPCR reaction was set up using SYBR Green PCR Master Mix (Bio-Rad, 1725121) containing 1 L cDNA