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Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity

Determination of intracellular reactive oxygen species (ROS) levels Total intracellular ROS levels were determined using a ROS assay kit (cat

CypD knockout (KO) in elderly mice showed better anti-aging effects, such as inhibition of mitochondrial swelling and a higher NAD+/NADH ratio, but further exploration is needed to explain the exact mechanism in heart tissue [38]