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Proc Natl Acad Sci USA 103(39):1430214306

MMPs (such as MMP-1, -2, -3, -7, -9, -13, 14, and ADAMTS-4) are upregulated in IDD and correlate with the severity of degeneration (Zhang C

10.3390/dna2040020 78 Lpez-OtnC.BlascoM

After washing the membranes four times with TBST buffer, they were incubated at room temperature for 2 h with anti-His tag antibody (1:2000, Abbkine) in TBST buffer