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RFP+ clones were separated via fluorescence activated cell sorting (FACS, by using FACS 7 Aria IIu (BD Biosciences, Franklin Lakes, United States))

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Furthermore, since a combination of inhibitors specific for several different classes of zinc-dependent HDAC families was required to phenocopy the broad-spectrum inhibitor TSA, it is likely that HDACs downstream of multiple regulatory pathways act redundantly in IL-10 regulation

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